Recently, Artificial Cultivation And Anti-tumor Activity of Ganoderma mbrekobenum was published in Sains Malaysiana by the Guangdong Institute of Microbiology. .Huiping Hu is the first author and corresponding author of the paper.
G. mbrekobenum is a new species of Ganoderma that was published in Ghana in 2016. Compared with Ganoderma lucidum, its appearance is reddish brown. The author named it "reddish brown Ganoderma lucidum" according to the original Swahili meaning. The specimens in this study were collected from eastern Tanzania and are the first to be reported in the region.
In this study, the ITS sequence of this specimen was obtained through molecular identification, and a phylogenetic tree was constructed with the existing varieties of Ganoderma species. Combined with macroscopic and microscopic morphology, it was confirmed to be G. mbrekobenum. Phylogenetic tree showed that G. mbrekobenum was more closely related to G. zonatum, G. boninense and G. australe, which similarly growed in tropical and subtropical regions. The cultivation period was 78 days. The biological conversion rate (16.3%) was slightly lower than that of Hanzhi No. 2 and Hunong No. 1 (19.3% and 19.8%), which are commonly used in the domestic market, but far more than some wild varieties, such as G. australe (12.8%) and G. leucocontextum (10.1-11.4%), It showed that G. mbrekobenum had a better potential for artificial cultivation. Its fruiting body was more uniform than that of the above two Ganoderma species, it was hard, strong resistance. Further studies showed that the ethyl acetate extract had a certain inhibitory effect on human hepatocellular carcinoma cell (HepG2), human human cancer cell (U87) and human breast cancer cell (MDA-MB-231) in vitro, and the inhibitory effect on breast cancer cell was the most obvious with IC50 of 115 μGmL-1. In addition, the composition determination showed that under the same cultivation conditions, the polysaccharide content of G. mbrekobenum was significantly higher than that of the two Ganoderma lucidum market species, which was 1.12%, while the composition of Ganoderma acid A was significantly lower than that of them, indicating that the triterpenoid composition of G. mbrekobenum was different from that of common Ganoderma lucidum species, and further research was needed. In this study, a new variety of Ganoderma was developed, and a comprehensive study on G. mbrekobenum was conducted for the first time, revealing the potential utilization value of other varieties in Ganoderma. In the next step, cultivation conditions can be optimized, and the in-depth study on effective ingredients can be carried out, so as to provide new variety resources for the discovery of potential medicinal fungi.